Here is a list of 49 American AI startups that secured at least $100 million in funding in 2025

The previous year marked a significant turning point for the AI sector both in the United States and globally. 

According to TechCrunch’s records, 49 startups secured funding rounds of at least $100 million in 2024. Among them, three companies closed multiple “mega-rounds,” and seven secured investments of $1 billion or more. 

What will 2025 bring? As we approach the last month of the year, 2025 has already equaled 2024 in the number of companies raising $100 million or more. In fact, this year has seen a notable increase in startups securing multiple rounds above $100 million compared to the previous year.  

Below is a list of all U.S.-based AI startups that have raised at least $100 million in 2025: 

November

• Anysphere, the company behind the widely popular coding platform Cursor, secured $2.3 billion in funding, giving it a $29.3 billion valuation. Announced on November 13, this marks their second funding round this year.  

• Parallel, a company developing web infrastructure for AI agents, landed a $100 million Series A investment announced on November 12. Index Ventures and Kleiner Perkins co-led the round.  

• Healthcare AI agent startup Hippocratic AI closed a $126 million Series C round, valuing the company at $3.5 billion. This was their second round this year, announced November 3 and led by Avenir Growth.  

October

• Fireworks AI, a platform enabling users to build AI apps with open source models, raised $250 million in a Series C round announced October 28, valuing the company at $4 billion.  

• Enterprise AI company Uniphore achieved a $2.5 billion valuation after a $260 million Series F round announced October 22. Investors included Snowflake Ventures, Nvidia, Databricks Ventures, and AMD.  

• Sesame, specializing in voice AI, secured $250 million in a Series B round co-led by Sequoia and Spark Capital. The round, announced October 21, also saw SignalRank participate.  

• Cambridge, Massachusetts-based OpenEvidence, which develops an AI chatbot for healthcare, completed its second funding round of 2025. The $200 million Series C, announced October 20, valued the company at $6 billion.  

• Lila Sciences, aiming to create a science superintelligence platform, announced its second funding round of 2025 on October 14. The $350 million Series A was co-led by Braidwell and Collective Global. 

• DeepSeek rival Reflection AI announced its second mega-round of the year on October 9. The $2 billion Series B valued the company at $8 billion and was led by Nvidia.  

• EvenUp, which develops AI for personal injury law, announced a $150 million Series E round that valued the company at over $2 billion on October 7. Bessemer led the round, with Lightspeed, Bain Capital, and SignalFire among the participants.  

September

• Periodic Labs, working on an AI scientist, announced a $300 million seed round on September 30. Felicis and Andreessen Horowitz led the round, joined by Nvidia, Lightspeed, and Khosla Ventures.  

• Cerebras Systems, an AI infrastructure provider, raised $1.1 billion in a Series G round, valuing the company at $8.1 billion. The round, announced September 30, was co-led by Fidelity and Atreides Management. 

• Modular announced a $250 million funding round on September 24. The round was led by US Innovative Technology Fund, with GV, Greylock, and General Catalyst also investing.  

• Distyl AI, which creates enterprise AI software, secured $175 million in a Series B round announced September 23. The round valued the company at $1.8 billion and included Khosla Ventures and Lightspeed.  

• AI infrastructure startup Upscale AI raised $100 million in a seed round co-led by Maverick Silicon and Mayfield. The round, announced September 17, also included StepStone Group, Stanford University, and Qualcomm Ventures.  

• Groq, an AI inference company, secured $750 million in a Series E round, valuing the company at nearly $6.9 billion. Disruptive led the round, announced September 17.  

• AI training company Invisible Technologies reached a $2 billion valuation after raising $100 million, announced September 16. Vanara Capital led the round, with Greycroft, Tallwoods Capital, and Freestyle Capital also participating.  

• Cognition AI, creator of the coding agent Devin, raised $400 million in a Series C round announced September 8. Founders Fund led the round, valuing the company at $10.2 billion. 

• AI infrastructure startup Baseten raised $150 million in a Series D round, valuing the company at $2.1 billion. The round, led by Bond, was announced September 5, with CapitalG, IVP, and Spark Capital also investing.  

• Bret Taylor’s customer service AI platform Sierra raised $350 million in a round led by Greenoaks Capital. Announced September 4, this round valued Sierra at over $10 billion.  

• You.com, a personalized AI search engine, raised $100 million in a Series C round led by Cox Enterprises. The round, announced September 3, valued the company at $1.5 billion.  

• AI research lab Anthropic completed its second round of 2025 in September. Anthropic announced a $13 billion Series F round on September 2, valuing the company at $183 billion. Iconiq, Fidelity, and Lightspeed led the round.  

August

• Healthcare and housing automation platform EliseAI raised $250 million in a Series E round, valuing the company at $2.2 billion. The round, announced August 20, was led by Andreessen Horowitz.
• Decart, an AI research lab, raised $100 million at a $3.1 billion valuation. The round, announced August 7, included Sequoia Capital, Benchmark, and Zeev Ventures.

July

• Generative media platform Fal secured $125 million in a Series C round led by Meritech Capital Partners. The round, announced July 31, valued Fal at $1.5 billion, with Salesforce Ventures, Shopify Ventures, Google AI Futures Fund, and others participating.
• Five-year-old Ambience Healthcare, developing an AI-powered healthcare operating system, raised $243 million in a Series C round led by Oak HC/FT and Andreessen Horowitz. Kleiner Perkins, OpenAI Startup Fund, Smash Capital, and others also joined the round.
• Reka AI, an AI research lab, raised $110 million in a Series B round announced July 22, valuing the company at $1 billion. Snowflake and Nvidia participated in the round.
• AI research lab Thinking Machines Lab confirmed a $2 billion seed round on July 15. Andreessen Horowitz led the round, with Nvidia, Accel, and AMD among the investors. The company is now valued at $12 billion.
• Cambridge, Massachusetts-based OpenEvidence, which is developing an AI search tool for clinicians, raised $210 million at a $3.5 billion valuation. The Series B round, announced July 15, was led by Kleiner Perkins and GV.
• Harmonic, building a mathematical reasoning engine, raised $100 million in a Series B round led by Kleiner Perkins. The round, announced July 10, valued the company at $875 million.

June

• Healthcare AI unicorn Abridge announced a $300 million Series E round, valuing the company at $5.3 billion. Andreessen Horowitz led the round, with Khosla Ventures also participating. This was Abridge’s second round in 2025.
• Harvey, which develops AI tools for the legal sector, announced its second $300 million round of 2025 on June 23. The Series E round, co-led by Kleiner Perkins and Coatue, brought the company’s valuation to $5 billion.
• Healthcare AI startup Tennr announced a $101 million Series C round led by IVP, with Lightspeed Venture Partners, GV, and Andreessen Horowitz among the investors. The round valued Tennr at $605 million.
• Enterprise search company Glean continues to attract investment. On June 10, the company announced a $150 million Series F round led by Wellington Management, with Sequoia, Lightspeed Venture Partners, and Kleiner Perkins also participating. Glean is now valued at $7.25 billion.
• Anysphere, the AI research lab behind coding tool Cursor, raised $900 million in a Series C round, valuing the company at nearly $10 billion. Thrive Capital led the round, with Andreessen Horowitz, Accel, and DST Global also investing.

May

• AI data labeling company Snorkel AI announced a $100 million Series D round on May 29, giving it a $1.3 billion valuation. Addition led the round, with Prosperity7 Ventures, Lightspeed Venture Partners, and Greylock also participating.
• LMArena, a widely used community benchmarking tool for AI models, raised $100 million in a seed round that valued the company at $600 million. The round, announced May 21, was co-led by Andreessen Horowitz and UC Investments, with Lightspeed Venture Partners, Kleiner Perkins, and Felicis also joining.
• Las Vegas-based AI infrastructure firm TensorWave announced a $100 million Series A round on May 14. Magnetar Capital and AMD Ventures co-led the round, with Prosperity7 Ventures, Nexus Venture Partners, and Maverick Silicon also investing.

April

• SandboxAQ completed a $450 million Series E round on April 4, valuing the AI model company at $5.7 billion. Investors included Nvidia, Google, and Ray Dalio, founder of Bridgewater Associates.
• Runway, which develops AI models for media production, raised $308 million in a Series D round announced April 3, valuing the company at $3 billion. General Atlantic led the round, with SoftBank, Nvidia, and Fidelity also participating.

March

• AI giant OpenAI raised a record-setting $40 billion round, valuing the company at $300 billion. The round, which closed March 31, was led by SoftBank, with Thrive Capital, Microsoft, and Coatue also investing.
• On March 25, Nexthop AI, an AI infrastructure company, announced a $110 million Series A round led by Lightspeed Venture Partners, with Kleiner Perkins, Battery Ventures, and Emergent Ventures also participating.
• Cambridge, Massachusetts-based Insilico Medicine raised $110 million for its generative AI drug discovery platform, as announced March 13. The Series E round, co-led by Value Partners and Pudong Chuangtou, valued the company at $1 billion.
• AI infrastructure company Celestial AI raised $250 million in a Series C round, valuing the company at $2.5 billion. The round, announced March 11, was led by Fidelity, with Tiger Global, BlackRock, and Intel CEO Lip-Bu Tan also participating.
• Lila Sciences raised $200 million in a seed round to develop a science superintelligence platform. Flagship Pioneering led the round, with March Capital, General Catalyst, and ARK Venture Fund also investing.
• Brooklyn-based Reflection.Ai, focused on building superintelligent autonomous systems, raised $130 million in a Series A round, valuing the one-year-old company at $580 million. Lightspeed Venture Partners and CRV led the round.
• AI coding startup Turing closed a $111 million Series E round on March 7, valuing the company, which partners with LLM firms, at $2.2 billion. Khazanah Nasional led the round, with WestBridge Capital, Gaingels, and Sozo Ventures also participating.
• Shield AI, an AI defense technology startup, raised $240 million in a Series F round that closed March 6. L3Harris Technologies and Hanwha Aerospace co-led the round, with Andreessen Horowitz and the US Innovative Technology Fund also investing. The round valued the company at $5.3 billion
• AI research and large language model company Anthropic raised $3.5 billion in a Series E round, valuing the company at $61.5 billion. The round, announced March 3, was led by Lightspeed, with Salesforce Ventures, Menlo Ventures, and General Catalyst also participating.

February

• Together AI, which develops open source generative AI and model development infrastructure, raised $305 million in a Series B round, valuing the company at $3.3 billion. The round, announced February 20, was co-led by Prosperity7 and General Catalyst, with Salesforce Ventures, Nvidia, Lux Capital, and others joining.
• AI infrastructure company Lambda raised $480 million in a Series D round announced February 19. The round, co-led by SGW and Andra Capital, valued the company at nearly $2.5 billion. Nvidia, G Squared, ARK Invest, and others also participated.
• Abridge, an AI platform for transcribing patient-clinician conversations, was valued at $2.75 billion in a Series D round announced February 17. The $250 million round was co-led by IVP and Elad Gil, with Lightspeed, Redpoint, and Spark Capital also investing.
• Eudia, an AI legal tech startup, raised $105 million in a Series A round led by General Catalyst. Floodgate, Defy Ventures, and Everywhere Ventures, along with other VC firms and angel investors, also participated. The round closed February 13.
• AI hardware startup EnCharge AI raised $100 million in a Series B round that also closed February 13. Tiger Global led the round, with Scout Ventures, Samsung Ventures, and RTX Ventures also investing. The Santa Clara-based company was founded in 2022.
• AI legal tech company Harvey raised $300 million in a Series D round, valuing the three-year-old company at $3 billion. Sequoia led the round, announced February 12, with OpenAI Startup Fund, Kleiner Perkins, Elad Gil, and others also participating.

January

• Synthetic voice company ElevenLabs raised $180 million in a Series C round, valuing the company at over $3 billion. The round, announced January 30, was co-led by ICONIQ Growth and Andreessen Horowitz, with Sequoia, NEA, Salesforce Ventures, and others also investing.
• Hippocratic AI, which develops large language models for healthcare, announced a $141 million Series B round on January 9. The round, led by Kleiner Perkins, valued the company at over $1.6 billion. Andreessen Horowitz, Nvidia, and General Catalyst also participated.

This article was updated on April 23, June 18, August 27, and November 26 to reflect additional deals.

This article has been revised to clarify that Abridge was founded in Pittsburgh, rather than being based there.